Escudo de la República de Colombia
Seguridad Alimentaria Nutricional S.A.N. Nariño

Scientific Publications

2015 Development and validation of a liquid chromatographic method to quantify sucrose, glucose, and fructose in tubers of Solanum tuberosum Group Phureja

A High Performance Liquid Chromatography (HPLC) method was developed and validated to quantify sucrose (non-reducing sugar), glucose, and fructose (reducing sugars) in raw tubers of Solanum tuberosum Group Phureja. Chromatographic analysis was performed using an AMINEX HPX 87H column, at 18 °C, linked to a refraction index detector, at 35 °C. The eluent was 10 mM sulfuric acid. The conditions established for the method provided an optimum separation of sugars, citric acid, and malic acid, with resolution values higher or equal to one. Among the four sugar extraction methods tested, the double 50% (v/v) aqueous methanol extraction gave the highest level of analytes. Recovery of this extraction method ranged between 94.14 and 99.77%. The HPLC method was validated for repeatability, reproducibility, linearity, and limits of detection, and quantification. Relative standard deviation was found to be lower than five, when testing repeatability and reproducibility, which is suitable considering a range of acceptability from 5.3 to 7.3. Additionally, the regression analyses supported the method linearity in a range of quantification from 3 to 100 mg/L with regression coefficients values greater than 0.998 for the three analytes. Limits of detection were 3.0 mg/L for the three sugars and limits of quantification were 2.0 mg/L for sucrose and 3.0 mg/L for glucose and fructose. Four Colombian commercial cultivars (Criolla Guaneña, Criolla Paisa, Criolla Galeras, and Criolla Colombia) and five landrace accessions from the Colombian Core Collection of Group Phureja were grown in the district of Usme (Bogotá) fields to analyze their sugar contents. Sucrose, glucose, and fructose contents were found ranging from 0.93 to 3.11 g/100 g tuber dried weight (DW), from 0.25 to 4.53 g/100 g tuber DW, and from 0.10 to 1.49 g/100 g tuber DW, respectively. Therefore, a high range in the variability of sugar contents was found among genotypes. However, the variability was low among technical replicates of the same genotype, revealing an accurate quantification of sugars in Group Phureja. This method can be used to assess the amount of reducing and non-reducing sugars accumulation in potato germplasm.

http://www.sciencedirect.com/science/article/pii/S1570023214006801

The Roles of Rural Women in the Department of Nariño, Colombia. Trends and Changes

This article presents reflections based on the work in “Spaces of Encounter” within the framework of the field schools -ECAS- with rural and indigenous potato growers in five municipalities of the Department of Nariño. It identified issues, roles of women and men in family life and in agricultural work as well as expectations and desire for change and projections in their community interactions, associated with food security for their families and communities. It especially deals with the interest of rural women to “prepare” for decision making in family and community life.

http://www.revistas.unal.edu.co/index.php/tsocial/article/view/47069/48375

Development and validation of a liquid chromatographic method to quantify sucrose, glucose, and fructose in tubers of Solanum tuberosum Group Phureja

A High Performance Liquid Chromatography (HPLC) method was developed and validated to quantify sucrose (non-reducing sugar), glucose, and fructose (reducing sugars) in raw tubers of Solanum tuberosum Group Phureja. Chromatographic analysis was performed using an AMINEX HPX 87H column, at 18 °C, linked to a refraction index detector, at 35 °C. The eluent was 10 mM sulfuric acid. The conditions established for the method provided an optimum separation of sugars, citric acid, and malic acid, with resolution values higher or equal to one. Among the four sugar extraction methods tested, the double 50% (v/v) aqueous methanol extraction gave the highest level of analytes. Recovery of this extraction method ranged between 94.14 and 99.77%. The HPLC method was validated for repeatability, reproducibility, linearity, and limits of detection, and quantification. Relative standard deviation was found to be lower than five, when testing repeatability and reproducibility, which is suitable considering a range of acceptability from 5.3 to 7.3. Additionally, the regression analyses supported the method linearity in a range of quantification from 3 to 100 mg/L with regression coefficients values greater than 0.998 for the three analytes. Limits of detection were 3.0 mg/L for the three sugars and limits of quantification were 2.0 mg/L for sucrose and 3.0 mg/L for glucose and fructose. Four Colombian commercial cultivars (Criolla Guaneña, Criolla Paisa, Criolla Galeras, and Criolla Colombia) and five landrace accessions from the Colombian Core Collection of Group Phureja were grown in the district of Usme (Bogotá) fields to analyze their sugar contents. Sucrose, glucose, and fructose contents were found ranging from 0.93 to 3.11 g/100 g tuber dried weight (DW), from 0.25 to 4.53 g/100 g tuber DW, and from 0.10 to 1.49 g/100 g tuber DW, respectively. Therefore, a high range in the variability of sugar contents was found among genotypes. However, the variability was low among technical replicates of the same genotype, revealing an accurate quantification of sugars in Group Phureja. This method can be used to assess the amount of reducing and non-reducing sugars accumulation in potato germplasm.

http://www.sciencedirect.com/science/article/pii/S1570023214006801

Nutritional Situation of Rural Communities in Nariño, Colombia 2013

Objective: To evaluate the nutritional situation by anthropometry of the rural families population aged 0 to 64 years in the municipalities of Pasto, Túquerres, Guachucal, Cumbal and Cuaspud in the Nariño Department (Colombia). Materials and Methods: As part of the research “Improving potato production as a food security contribution of native communities in Nariño, Colombia”, aged October and December in 2013, an initial diagnosis of the food security situation in farmer families was conducted, through direct visits to the households selected in the sample, where in the household members, after standardization of field team, the anthropometric measures of weight and height were taken, using electronic scales, infantometers and stadiometers. Conclusions: A significant stunting was reported in children up to 7 years. In children aged 0 - 5 years group and children aged 5 - 17 years group, the results exceed by more than double the department data (according to National Survey of the Nutritional Situation in Colombia ENSIN 2010), and almost three times the national data. The underweight was generally low in all the analysed groups, although concerned the significant overweight percentage. In children aged 0 - 5 years group, the overweight data (above 2 SD) exceeded twice the department and national data according to the ENSIN 2010, although in the children aged 5 - 17 years group, the overweight and obesity was lower than the departmental and national data. In adults, the results reported that one of each two people in the range of 18 to 64 years in the five municipalities had excess weight (overweight or obesity) which was similar data respected to the department and national data. Additionally, it was noted that 18% of households were found among adults with overweight, and simultaneously, children with stunting, this situation has been called double burden of malnutrition and this is an aspect that must be analysed to identify intervention strategies.

http://www.scirp.org/journal/PaperInformation.aspx?PaperID=49218#.VH94uD...

2014 - Identification of Late Blight Resistance-Related Metabolites and Genes in Potato through Nontargeted Metabolomics

Doddaraju Pushpa - Kalenahalli N. Yogendra - Raghavendra Gunnaiah - Ajjamada C. Kushalappa - Agnes Murphy

Late blight of potato (Solanum tuberosum) caused byPhytophthora infestanssignificantly reduces the productivity of potato around the world. Resistance to late blight in potato is either qualitative or quantitative. Qualitative resistance governed by race-specific singleRgenes is well characterized and gives complete resistance, but is not durable.

Ver en http://link.springer.com/article/10.1007%2Fs11105-013-0665-1

2014 - Quantitative resistance in potato leaves to late blight associated with induced hydroxycinnamic acid amides

Kalenahalli N. Yogendra - Doddaraju Pushpa - Kareem A. Mosa - Ajjamada C. Kushalappa - Agnes Murphy - Teresa Mosquera

Late blight is a serious economic threat to potato crop, sometimes leading to complete crop loss. The resistance in potato to late blight can be qualitative or quantitative in nature. Qualitative resistance is not durable. Though quantitative resistance is durable, the breeding is challenging due to polygenic inheritance.

Ver en http://link.springer.com/article/10.1007%2Fs10142-013-0358-8    

2013 - Metabolo-proteomics to discover plant biotic stress resistance genes

Ajjamada C. Kushalappa and Raghavendra Gunnaiah

Plant Science Department, McGill University, Sainte-Anne-de-Bellevue, Quebec, H9X 3V9, Canada.
Plants continuously encounter various environmental stresses and use qualitative and quantitative measures to resist pathogen attack. Qualitative stress responses, based on monogenic inheritance, have been elucidated and successfully used in plant improvement.

 

Ver en http://www.sciencedirect.com/science/article/pii/S1360138513001039   

2012 - Potatoes and Human Health

MARY ELLEN CAMIRE, STAN KUBOW and DANIELLE J. DONNELLY

The potato (Solanum tuberosum L.) tuber follows only rice and wheat in world importance as a food crop for human consumption. Cultivated potatoes have spread from the Andes of South America where they originated to 160 countries around the world. Consumption of fresh potatoes has declined while processed products have increased in popularity. As the potato becomes a staple in the diets of an increasing number of humans, small differences in potato nutritional composition will have major impacts on population health.

 

 

 

PDF - Potatoes and Human Health

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